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KMID : 0364820150510040364
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Korean Journal of Microbiology
2015 Volume.51 No. 4 p.364 ~ p.373
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Isolation and characterization of marine bacteria with alginate degrading activity
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Yoon Young-June
Kim Jung-Wan
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Abstract
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As an effort to utilize alginate, 103 bacterial isolates that were positive for the alginate lyase activity were isolated from various clams and seawater samples collected in Incheon coastal area. Among them, 3 strains (M1-2-1, M6-1, and C8-15) were finally selected for further analysis based on their activities at higher levels than others. These isolates were all Gram-negative and rod shaped halophilic bacteria with motility. According to their physiological and biochemical properties as well as DNA sequence of their 16S rRNA genes, M1-2-1 and M6-1 were identified as a member of genus Pseudoalteromonas and C8-15 belonged to genus Vibrio. They exhibited the alginate degrading activity at the maximal level when they were cultured in APY broth for 6?8 h at 25°C. Both their growth and the enzyme activity were greatly enhanced when NaCl was added to the growth medium. The crude alginate lyases from the supernatants of the bacterial cultures showed the highest activity at 45°C and pH 7.0?8.0. M1-2-1 and M6-1 produced 2.723 and 1.976 g/L of reducing sugar from alginate, respectively, suggesting that they have potential for commercial application.
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KEYWORD
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Pseudoalteromonas sp., Vibrio sp., alginate, alginate lyase, brown algae
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